Extended Data Fig. 2: NicA2 is a dimer in solution.
From: A cytochrome c is the natural electron acceptor for nicotine oxidoreductase
Previous work has described NicA2 as a monomer using size exclusion chromatography14,15. To determine the quaternary structure of NicA2 in our buffer conditions, a solution of NicA2 at 20 μM (monomer concentration) was subjected to analysis by sedimentation velocity AUC at a rotor speed of 44,000 rpm while monitoring 450 nm. Data were analyzed using UltraScan 4, version 4.0. One species predominated in solution, with a sedimentation coefficient of ~5.45 and an apparent molecular weight of ~115 kDa. The expected molecular weight for the NicA2 monomer is 53.13 kDa, indicating that NicA2 is a homodimer in solution under these conditions. This experiment was independently repeated twice with similar results.
