Extended Data Fig. 3: NicA2 rapidly forms a complex when titrated with the non-catalytic nicotine analog myosmine.
From: A cytochrome c is the natural electron acceptor for nicotine oxidoreductase
a, Tryptophan fluorescence was used to quantify binding of myosmine as previously performed9. Traces from a stopped-flow experiment where oxidized NicA2 was mixed with varying concentrations of myosmine demonstrate a rapid binding event, occurring within the dead time (1 ms) of the instrument. b, Averaged fluorescence values of 5 traces per myosmine concentration were fit to determine the Kd for myosmine binding at 268 ± 3 μM (s.e.m.).
