Extended Data Fig. 4: Binding, trafficking and signaling properties of PTHH9A.
From: Allosteric interactions in the parathyroid hormone GPCR–arrestin complex formation
a, Co-immunoprecipitation of HA-tagged PTHR and β-arrestins from HEK293 cells after stimulation with PTHWT. Stimulation with PTHH9A does not immunoprecipitate β-arrestin with PTHRHA. b, Averaged time courses of PTHR internalization after 60 s stimulation with 100 nM PTHWT or PTHH9A in HEK293 cells stably expressing PTHRSEP. Data represent the mean ± s.d. of N = 2 experiments with n = 18 cells per experiment (independent biological replicates). c, Radioligand competition assays of PTHWT and PTHH9A binding to PTHR R0 (G protein-free) conformation. d, Radioligand competition assays of PTHWT and PTHH9A binding to PTHR RG conformation. For c, d, data are averaged from N = 4 and N = 5 independent experiments, respectively. Square points and error bars represent mean ± s.e.m. Individual data points are also shown.
