Extended Data Fig. 1: Proteome-wide mapping of drug-induced protein abundance changes in five cell lines.
From: A mass spectrometry-based proteome map of drug action in lung cancer cell lines
a, The heatmap shows the pairwise Pearson correlations (color-coded from 0.95–1) of proteomes from one 96-well plate (the average pairwise Pearson R is 0.983; data is based on 96 biologically independent samples). b, To define MS1 mass-to-charge ratio (m/z) segments, the m/z ion intensity was recorded over a 120 min gradient, summed up and distributed into 1 Da bins (only z > 1 was considered). After calculating the percentage of intensity per 1 Da bin, the m/z range was distributed into segments such that the overall intensity in each segment is equal. The graph shows an example for the distribution of the ion density into three segments (S): S1 = m/z 350–525; S2 = m/z 525–695; S3 = m/z 695–1,200. c, Heatmap showing the number of regulated proteins per inhibitor and cell line (two-sided t-test with permutation-based FDR < 0.05; n = 12 (DMSO) and 3 (each inhibitor) biologically independent samples).
