Extended Data Fig. 5: Constructing intracellular acoustic sensor genes for dynamic monitoring of protease activity and circuit-driven gene expression.
From: Acoustic biosensors for ultrasound imaging of enzyme activity
a, Normalized pressure-sensitive optical density at 600 nm of WT Nissle cells expressing either ARGWT or ASGClpXP. The legend lists the midpoint collapse pressure for each cell type (±95% confidence interval) determined from fitting a Boltzmann sigmoid function (N = 5 biological replicates and 8 total replicates for ASGClpXP; N = 3 biological replicates for ARGWT and 6 total replicates). b, Representative ultrasound images of WT Nissle cells expressing either ARGWT or ASGClpXP at OD600nm 1.5 (N = 4 biological replicates and the number of total replicates is 10). c, Scatter plots showing x-AM/B-mode ratio as a function of applied acoustic pressure for WT Nissle cells expressing either ARGWT or ASGClpXP at OD600nm 1.5 (N = 4 biological replicates and the number of total replicates is 10). d, Scatter plots for Fig. 4b, N = 3 biological replicates. e, f, Scatter plots showing the ratio of x-AM to B-mode acoustic signal as a function of acoustic pressure for all the replicate samples used in the x-AM voltage ramp experiments for ΔclpXP Nissle cells expressing ASGClpXP and araBAD driven clpXP, with or without L-arabinose induction (e) and WT Nissle cells expressing ASGClpXP and pTet-TetO driven WT gvpC, with or without aTc induction (f). N = 3 biological replicates, with each N having 3 technical replicates for (e) and N = 5 biological replicates for (f). Individual dots represent each N and solid line represents the mean of all the replicates for a, c–f.
