Extended Data Fig. 7: Ultrasound imaging of bacteria expressing acoustic sensor genes in the gastrointestinal tract of mice.
From: Acoustic biosensors for ultrasound imaging of enzyme activity
a, Schematic illustrating two orientations of the wild type (WT) E. coli Nissle cells expressing ARGWT or ASGClpXP introduced into the mouse colon as a hydrogel. b, c, Representative transverse ultrasound images of the colon for two mice used in the in vivo imaging experiments, with orientation #1 (b) and with orientation #2. (c). Cells are injected at a final concentration of 1.5E9 cells ml-1. B-mode signal is displayed using the bone colormap and x-AM signal is shown using the hot colormap. Color bars represent B-mode and x-AM ultrasound signal intensity in the dB scale. Scale bars represent 2 mm. d, e, B-mode and xAM contrast-to-noise ratio (CNR) in vivo, for WT Nissle cells expressing ARGWT or ASGClpXP in orientation #1 (d) and orientation #2. (e). N = 5 mice for orientation #1 (b, d) and N = 4 mice for orientation #2 (c, e). Error bars indicate SEM. P = 0.0014 for x-AM signal from cells expressing ASGClpXP versus the ARGWT control in orientation #1, and P = 0.0016 for that in orientation #2. P = 0.0570 for B-mode signal in orientation #1 and P = 0.3445 in orientation #2. P-values were calculated using a two-tailed paired t-test. Individual dots represent each N and horizontal line indicates the mean.
