Extended Data Fig. 9: Absence of memory effect from imaging at sequentially increasing acoustic pressure.
From: Acoustic biosensors for ultrasound imaging of enzyme activity

Ratio of sensor-specific signal (xAM/B-mode) acquired at the indicated acoustic pressures in the process of voltage ramping (comprising 36 points from 458 kPa to 1.6 MPa) or stepping the transducer output directly to corresponding pressure in a single step, for WT Nissle cells expressing either ARGWT or ASGClpXP. N = 3 biological replicates, with each N having 3 technical replicates. Individual dots represent each replicate, and the thick horizontal line indicates the mean. Error bars indicate SEM derived from biological replicates (see Online Methods).