Extended Data Fig. 1: Engineering an acoustic sensor of TEV endopeptidase activity.
From: Acoustic biosensors for ultrasound imaging of enzyme activity
a, Coomassie-stained SDS-PAGE gel of OD500nm-matched samples of GVWT incubated with dTEV and TEV protease, before and after buoyancy purification (labeled pre b.p. and post b.p., respectively). N = 3 biological replicates. b, Scatter plots showing normalized OD500nm of GVSTEV as a function of hydrostatic pressure. (N = 3 biological replicates for GVSTEV + TEV and N = 4 for GVSTEV + dTEV.) c, Scatter plots showing the ratio of nonlinear (x-AM) to linear (B-mode) ultrasound signal as a function of applied acoustic pressure for all the replicate samples used in the x-AM voltage ramp imaging experiments for GVSTEV. N = 3 biological replicates and total number of replicates is 8. d, Scatter plots showing normalized OD500nm of GVWT as a function of hydrostatic pressure. (N = 3 biological replicates for GVWT + dTEV and N = 4 for GVWT + TEV.) e, Representative ultrasound images of agarose phantoms containing GVWT incubated with TEV or dTEV protease at OD500nm 2.2. The B-mode image was acquired at 132kPa and the x-AM image at 569 kPa. Similar images acquired for N = 3 biological replicates, with each N consisting of 3 technical replicates. CNR stands for contrast-to-noise-ratio, and color bars represent relative ultrasound signal intensity on the dB scale. Scale bars represent 1 mm f, Scatter plots showing the ratio of nonlinear (x-AM) to linear (B-mode) ultrasound signal as a function of applied acoustic pressure for all the replicate samples used in the x-AM voltage ramp imaging experiments for GVWT. N = 3 biological replicates, with each N consisting of 3 technical replicates. Solid curve represents the mean of all the replicates.
