Extended Data Fig. 1: Site-specific Cy3 and/or Cy5 labeling of ribosomes purified from wildtype versus genomic mutant strains.
From: Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes
SDS-PAGE analysis of ribosomal proteins derived from 30 S or 50 S subunits isolated from the wildtype (wt) strain (Lanes 1 and 2) or the IR1, MT1, HS1, and HS2 mutant strains (Lanes 4-8) and reacted with DBCO-derivatized Cy3 and/or Cy5 fluorophores as shown in Fig. 3. Left panel shows visible light scan of Coomassie-stained gel. Middle and right panels show fluorescence emission scans of pre-Coomassie-stained gel using excitation wavelengths of 532 nm for Cy3 (Middle Panel) and 635 nm for Cy5 (Right Panel). The position at which each labeled ribosomal protein is expected to run on the SDS-PAGE gel was determined using a standard protein molecular weight ladder, and is indicated along the right side of the figure. The experiment shown here was replicated a total of three times, with similar results each time.
