Extended Data Fig. 2: Idealized EFRET versus time trajectories generated using Bayesian inference-based hidden Markov modeling.
From: Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes
Representative Cy3- and Cy5 fluorescence intensity versus time trajectories (Top Sub-Panel) and corresponding EFRET versus time trajectories (Bottom Sub-Panel) for smFRET experiments performed on ribosomal complexes assembled using Cy3- and/or Cy5-labeled 30 S and/or 50 S subunits isolated from the (a and b) HS1, (c) MT1, and (d) IR1 strains as shown in Fig. 4. In the Cy3 and Cy5 fluorescence intensity versus time trajectories, the Cy3 and Cy5 fluorescence intensities are shown as green and red curves, respectively. In the EFRET versus time trajectories, the EFRET is shown as blue curves. The idealized EFRET versus time trajectory is shown as black lines.
