Extended Data Fig. 5: Binding and functional data for the HHAT_p8F neoepitope, WT peptide, and variants.

a, SPR binding curves for 302TIL TCR recognition of each peptide-HLA-A*02:06 complex. Each experiment consists of duplicate injections that were fit simultaneously. For each peptide, one series of duplicate injections and associated fits are shown. Experiments with peptides other than the neoepitope were fit together with a separate neoepitope titration to ensure accurate determination of weak K_D values as described in ref. ²⁹. K_D values are indicated and reflect the averages and standard deviations of the number of independent experiments shown in Table 1. nbd, no binding detected; p6B, position 6 substituted with the Trp analogue Bta; RU, resonance unit. b, IL-2 production by 302TIL-transduced Jurkat 76 cells when co-cultured with HLA-A*02:06⁺ T2 cells pulsed with 100 μM peptide. VLF is an irrelevant negative control peptide (VLFGFTNFL). Data points are from three replicates in two independent experiments. Bars show the average of all six data points.