Extended Data Fig. 1: TA-enhanced signaling effect is also observed in SRE, NFAT, and NFκB gene expression assays.
From: G12/13 is activated by acute tethered agonist exposure in the adhesion GPCR ADGRL3
a, SRE b, NFκB and c, NFAT. For all gene response elements (SRE, NFAT, and NFκB) signaling was increased significantly when the entire N-terminal fragment up to the GPS cleavage site (CTF) was removed; FL receptor also showed some activity in SRE (comparable to CRE in Fig. 1). Luminescence in (a-c) was measured for a range of increased receptor cDNA concentrations ∼24 h after transfection in HEK293T cells. All data points are normalized to an empty vector control. Data are presented as mean values ±SEM from 3 independent experimental replicates.
