Extended Data Fig. 4: Compound BT5 does not bind covalently to NSD1 C2062A SET domain. | Nature Chemical Biology

Extended Data Fig. 4: Compound BT5 does not bind covalently to NSD1 C2062A SET domain.

From: Covalent inhibition of NSD1 histone methyltransferase

Extended Data Fig. 4

a, MS spectra of 1 μM wild-type NSD1 SET domain incubated with DMSO (left) or 16 μM BT5 (right) for 2 h showing 51% covalent engagement with BT5. b, MS spectra of 1 μM NSD1 C2062A SET domain incubated with DMSO (left) or 16 μM BT5 (right) for 2 h. No covalent engagement of BT5 is observed (expected mass 30,066 Da). Representative spectra shown are from two independent experiments (n = 2).

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