Extended Data Fig. 5: Treatment with BT5 lacks engagement with NSD2 and NSD3 in cells.

CETSA assay in HEK293T cells transfected with FLAG-NSD2 SET (top) or FLAG-NSD3 SET (bottom) constructs treated with DMSO or 5 μM BT5. Cell lysates were incubated for 3 min at indicated temperatures. Experiments were performed two times.