Extended Data Fig. 6: Effects of Cys on the 5 production in various culture media.
From: Mutanofactin promotes adhesion and biofilm formation of cariogenic Streptococcus mutans
a, Measurement of 5 production of Smu57 in biofilm medium supplemented with Cys at different concentrations. LC–HRMS extracted ion chromatogram traces of the metabolic extracts of Smu57 under different culture conditions were shown. EIC + = 698.25 ± 0.01, which corresponds to 5. b,c, Screening the optimal culture condition for 5 production. (b) A comparison of the yield of 5 from brain heart infusion (BHI) medium, BHI + Phe (1 mg/mL), BHI + Cys (1 mg/mL), and BHI + Phe+Cys (1 mg/mL for both amino acids). (c) A comparison of the yield of 5 from BHI medium supplemented with Cys at different concentrations. Results are presented as mean ± s.d. Data are representative of n = 3 independent experiments. Values with different letters represent significant differences (P < 0.05) determined by one-way ANOVA with Duncan’s test.
