Extended Data Fig. 9: Quantification of biofilms formed by cocultures of wild-type muf– strains with Smu56.
From: Mutanofactin promotes adhesion and biofilm formation of cariogenic Streptococcus mutans
a, Crystal violet biofilm assay (OD 575) was employed for biofilm quantification. b, Images of typical biofilms formed by different co-culture samples. Scale bar, 1 cm. c, Colony-forming units (CFUs) of indicated strains grown in co-culture biofilms, which are shown in a,b, were counted. This result shows that Smu56 coexisted with all selected wild-type muf– strains. Although biofilm formation is an important microbial competition factor, Smu56 formed strong biofilm via producing compound 5, which could be hijacked by its neighboring muf– S. mutans strains. a,c, Results are presented as mean ± s.d. Data are representative of n = 3 independent experiments. a, Significance was assessed using one-sided Student’s t-test.
