Extended Data Fig. 3: Confocal laser scanning microscopy (CLSM) images of stained biofilms.
From: Mutanofactin promotes adhesion and biofilm formation of cariogenic Streptococcus mutans
The biofilms formed by Smu57 and its Δmuf mutant were measured by CLSM after staining with a LIVE/DEAD BacLight Bacterial Viability Kits (SYTO-9 and propidium iodide). Green fluorescence represents viable bacteria and red fluorescence represents affected bacteria. The conventional anticaries agent chlorhexidine (5 μM) was used as a positive control. The experiment was repeated two independent times with reproducible findings. Scale bar, 20 µm.
