Extended Data Fig. 5: High-resolution and tandem MS of the thioamidated McrA peptide. | Nature Chemical Biology

Extended Data Fig. 5: High-resolution and tandem MS of the thioamidated McrA peptide.

From: Functional elucidation of TfuA in peptide backbone thioamidation

Extended Data Fig. 5

a, The thioamidated McrA peptide (GG-RLGFYGYDLQD) was characterized by HRMS. b, m/z 1,476.66 was subjected to CID with assigned ions indicated in tabular form. c, MS/MS spectrum confirming the location of the +16 Da mass change to the central Gly residue. Thioamide bond cleavage was not observed under the applied CID conditions.

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