Extended Data Fig. 6: TfuA catalyzes ThiS-COSH hydrolysis to generate sulfide.
From: Functional elucidation of TfuA in peptide backbone thioamidation
Quantification of sulfide (a) and MtThiS-COSH (b) via fluorescence detection at different time points of the reaction between MtThiS-COSH (150 μM) and MtTfuA of various concentrations. Sulfide concentrations were measured via reaction with 7-amino-4-methylcoumarin followed by fluorescence quantification. ThiS-COSH concentrations were determined using the LRSA-based assay. Data are presented as mean values ± s.d. (n = 3 independent experiments). c, Fluorescence quantification of MtThiS-COSH at different time points after reaction with 50 μM MtYcaO, 5 mM ATP, and McrA peptide of various concentrations. Data are presented as mean values ± s.d. (n = 3 independent experiments).
