Extended Data Fig. 7: McThiS 1H,15N-HMQC spectra upon MtTfuA titration.
From: Functional elucidation of TfuA in peptide backbone thioamidation
a, An overlay of McThiS 1H,15N-HMQC spectra in the presence (red) and absence (black) of MtTfuA (2 equiv.). Residues with substantial chemical shift perturbations are labeled. b, Zoomed regions of the spectrum showing McThiS residues with the largest chemical shift changes. The MtTfuA: McThiS ratio varies from 0:1 to 6:1 equiv. (MtTfuA concentrations vary from 110 μM to 1.2 mM).
