Extended Data Fig. 8: SDS-PAGE analysis of AzoYcaO co-purifying with His-AzoTfuA.
From: Functional elucidation of TfuA in peptide backbone thioamidation
(a) A Coomassie-stained SDS-PAGE gel showing untagged AzoYcaO (BAI72909.1) and His6-AzoTfuA (BAI72908.1) from Azospirillum sp. B510 co-expressed heterologously and co-purified using Ni-NTA affinity chromatography. In-gel trypsin digestion and subsequent MS analysis was performed to confirm the identity of the suspected His6-AzoTfuA (b) and AzoYcaO (c) bands. MALDI-TOF mass spectra of the tryptic peptides are labeled with subscripts corresponding native amino acid sequence covered. The identified tryptic fragments cover ~40% of the total protein sequence for both proteins.
