Extended Data Fig. 9: The presence of ATP alters the outcome of ThiS-COSH hydrolysis.
From: Functional elucidation of TfuA in peptide backbone thioamidation
a, Fluorescence quantification of MtThiS-COSH (initially 200 μM) upon reaction with MtTfuA (3 μM), MtYcaO (3 μM), and McrA peptide (100 μM), in the presence (red circles) or absence (black squares) of ATP (5 mM). Data are presented as mean values ± s.d. (n = 3 independent experiments). An exponential decay model was used to fit the data. b, Fluorescence quantification of sulfide production for MtThiS-COSH control and reactions in panel a at 140 min. Individual data points (n = 3 independent experiments) and mean values (lines) are presented. c, MALDI-TOF mass spectra of the McrA peptide from reactions in panel a at 140 min.
