Extended Data Fig. 1: TfuA-associated RiPP structures and TfuA sequence analysis.
From: Functional elucidation of TfuA in peptide backbone thioamidation
a, Representative thioamitide structures with thioamide moiety shown in red. b, A sequence similarity network (SSN) of the TfuA protein family PF07812 (n = 2,042 sequences) was generated with protein sequences 100% identical are conflated to a single node, and an alignment score of 60 (BLAST-P expectation value of 10-60) was used as the edge cut-off. Background is shaded based on taxonomy: archaea (orange), Proteobacteria (blue), Actinobacteria (pink), Cyanobacteria (purple), Firmicutes (green), and others (gray). Nodes representing TfuA proteins encoded within ten open-reading frames of a ThiS homolog are black. c, Gene neighborhood diagrams for selected methanogens encoding TfuA near ThiS. NCBI accession identifiers are given for the TfuA proteins.
