Extended Data Fig. 4: The foci formed by BLADE-sfGFP in the dark are aggregates and are due to the VVD moiety.
From: Engineering AraC to make it responsive to light instead of arabinose
a, GFP fluorescence intensity measured in E. coli MG1655 cells transformed with a modified pBLADE in which BLADE was C-terminally fused with sfGFP grown for 4h in the dark or under 460 nm light (5 W/m2) light. The individual data points are the mean values of 10,000 single-cell flow cytometry events. b, Left, representative images of a fluorescence recovery after photobleaching (FRAP) experiment. Scale bar, 5 μm. Right, Quantification of the recovery in three independent FRAP experiments. c, Quantification of the number of cells showing aggregates for the indicated constructs and conditions. From left to right, P = 0.00451 and P = 0.09117. Not significant (ns), P < 0.05; double asterisk (**), P < 0.01. P-values P were calculated by the two-tailed, homoscedastic Student’s t-test. Values represent mean ± SD of n = 3 independent experiments. Total number of cells analyzed: n = 670 (VVD-AraCDBDWT-sfGFP), and n = 473 (VVDC108A-AraCDBD-sfGFP).
