Extended Data Fig. 6: Setup for the characterization of BLADE TFs.
From: Engineering AraC to make it responsive to light instead of arabinose
a, Light induction setup for 96-well microtiter plates containing a panel of 96 light emitting diodes (LEDs), 3D-printed holders and a metal plate for heat dissipation. The setup comprises a custom-made printed circuit board (PCB) with 96 individual LEDs of three different wavelengths (red, green and blue). Each LED can be controlled individually using a microcontroller, enabling the exposure of each well to the same light intensity, a crucial aspect for the characterization of the chimeric TFs. A milled metal plate placed in between the PCB and the LEDs dissipates the heat produced by the light induction device. A 3D-printed microplate adapter on top of the metal plate allows for the precise positioning of the 96-well plate. The light intensity of the device was calibrated using a Thorlabs PM100USB Power and Energy meter connected with a Thorlabs S170C Microscope Slide Power Sensor. b, Domain composition of the engineered light-inducible dimerization domain (VVD and VfAu1)-AraC(DBD) fusion constructs. ‘Linkers’ indicate a library of different synthetic linkers that were tested between the two domains.
