Extended Data Fig. 8: IPTG-dose response curves for the VfAu1-AraC chimeric constructs.
From: Engineering AraC to make it responsive to light instead of arabinose
IPTG-dose response curves of cells transformed with the single plasmid bearing the indicated VfAu1-AraC fusions and mCherry under control of PBAD deprived of all upstream regulatory elements. Cells were either kept in the dark, or illuminated with saturating blue light (465 nm; 3.85 W/m2). The lines connect the mean mCherry fluorescence values of three individual samples, which are shown in blue squares for light induced samples and black dots for the dark control. For each condition, at least n = 3 individual experiments were performed, which are shown as individual points.
