Extended Data Fig. 9: IPTG- and light-dose response of AraC(DBD)::G4S::VVD.
From: Engineering AraC to make it responsive to light instead of arabinose
a, Optical density of E. coli MG1655ΔaraC cells expressing AraC(DBD)::G4S::VVD grown for 18h at 37 °C in a medium containing 2% arabinose and the indicated IPTG concentrations either in the dark or under 465 nm light (3.85 W/m2). b, Optical density of E. coli MG1655ΔaraC cells expressing AraC(DBD)::G4S::VVD grown for 17h20′ at 37 °C in a medium containing 2% arabinose and the indicated IPTG concentrations either in the dark or under 465 nm light of the indicated intensity. Bars represent mean ± SD of n = 3 biological replicates. Each data point shown in the bar plots represents a data point in a time course with measurements every 40 min, of which the average of the first three measurements was subtracted from all following time points to adjust for small differences in sample volume and inoculum. All time courses are shown in Supplementary Figs. 14 and 18.
