Extended Data Fig. 4: Development and characterization of biotinylated RING1B probe compounds.
From: Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
a, structures of RB-3-biot and RB-nc-biot. b, 1H-15N HSQC spectra of 60 μM 15N RING1B-BMI1f (green) titrated with 60 μM RB-3-biot or RB-nc-biot (red) and 120 μM RB-3-biot or RB-nc-biot (blue). c, Pull-down from HEK293T cells with RB-3-biot or RB-nc-biot followed by detection of RING1B or BMI1 using Western Blot analysis. Two controls were used in the pull-down assay, DMSO or biotinylated histone H3 peptide (biotin-H3), to assess non-specific binding. Assays were repeated two times.
