Extended Data Fig. 5: RB-3 inhibits H2Aub in cancer cell lines.
From: Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
a, Evaluation of BMI1 and RING1B knockdown, and treatment with RB-3 in K562 cells. Left western blot analysis in K562 cells transfected with BMI1/RING1B siRNAs for 4 d. Right immunoblots of K562 cells treated with indicated doses of RB-3 for 4 d. Representative blots of two independent experiments are shown. b, Evaluation of BMI1 and RING1B knockdown, and treatment with RB-3 in HeLa cells. Left Western blot in HeLa cells transfected with BMI1/RING1B siRNAs for 4 d. Right western blot analysis in HeLa cells treated with indicated doses of RB-3 for 4 d. Representative blots of two independent experiments are shown. c, Activity of RB-3 and RB-nc in MOLM13 cells treated for 4 d with indicated doses of compounds. Representative blots of two independent experiments are shown. d, Activity of RB-3 in MV4;11 cells treated for 4 d. Representative blots of two independent experiments are shown.
