Extended Data Fig. 8: Comparison of the activity of PTC209 and RB-3 in TEX cells.
From: Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
a, Graph showing effect of PTC209 on TEX cells proliferation. TEX cells were treated with the indicated concentrations of PTC209 for 4 days and processed for cell proliferation analysis. Experiment was performed 2 times, GI50 is mean ± s.d. b, western blot showing levels of H2Aub, H2Bub, H2A, H3 and BMI1 in TEX cells treated for 4 days with indicated doses of RB-3 (left) and PTC209 (right). Representative blots from two independent experiments. c, qRT–PCR indicating levels of C/EBPα upon 4 d treatment with indicated doses of RB-3 and PTC209. Representative data from two replicates. d, qRT–PCR indicating levels of CD34 upon 4 d treatment with indicated doses of RB-3 and PTC209. Representative data from two replicates.
