Extended Data Fig. 9: RB-3 has no effect on normal CD34⁺ cells.

a, Quantitation of colony numbers (CFU) upon treatment of cord blood CD34⁺ cells from healthy donors with RB-3. Experiments were performed two times and representative data are mean ± s.d. and analyzed by two-tailed t-test; ns – not significant. b, Population of CD34⁺ cells upon treatment with RB-3. Experiments were performed two times and representative data are mean ± s.d. and analyzed by two-tailed t-test; ns – not significant. c, Flow cytometry analysis of CD34 and CD38. Representative histograms of two independent experiments. d, Effect of RB-3 on various populations of mature cells with representative colony pictures. CFU-GM, granulocyte–macrophage progenitors; CFU-GEMM, oligopotential progenitors; BFU-E, burst-forming unit-erythroid cells; CFU-E, colony-forming unit-erythroid cells. Experiments were performed two times and representative data are mean ± s.d. and analyzed by two-tailed t-test; ns – not significant. e, Cell morphology of CD34⁺ cells treated with RB-3 analyzed by Wright Giemsa staining. Representative slides are shown from two independent experiments.