Extended Data Fig. 6: Affinities of anti-RBD nanobodies determined by surface plasmon resonance (SPR) or EC50 measurements.
From: Rapid generation of potent antibodies by autonomous hypermutation in yeast
SPR or EC50 binding curves are shown for each anti-RBD nanobody characterized in this study. For SPR measurements (Y-axis = Response), kinetic fits are shown where available and steady-state affinity fits are shown for nanobodies for which the on and off rates could not be determined. For EC50 affinities (Y-axis = Normalized Fluorescence), binding of yeast-displayed nanobodies by each concentration of RBD was determined in biological triplicate (n = 3, error bars represent ± s.d.) and EC50s were determined by fitting each binding curve.
