Extended Data Fig. 9: Gating strategy for singlets in all FACS experiments.
From: Rapid generation of potent antibodies by autonomous hypermutation in yeast
(left) Forward scatter (horizontal axes) versus side scatter (vertical axes) of a representative population of yeast cells. Red circle represents cells passing the gate. (right) Forward scatter area (horizontal axes) vs. forward scatter height (vertical axes) gating of cells that passed through the previous gate. Green boundary represents cells passing the gate. For all FACS experiments, only cells sorted through both gates were used in nanobody expression and binding gates and.
