Extended Data Fig. 2: Evolution of anti-AT1R nanobodies by AHEAD.
From: Rapid generation of potent antibodies by autonomous hypermutation in yeast
a, Contributions of individual mutations fixed during the evolution of AT110 by AHEAD. Affinity (EC50) of each nanobody for AT1R was determined by measuring binding of yeast-displayed nanobodies to each concentration of AT1R-angiotensin II complex (X-axis) in a single replicate and fitting the resulting binding curve. b, Amino acid sequence of AT110 and evolved variants. Mutations that were discovered using AHEAD are underlined in bold. Mutations that were discovered in a previous AT110 evolution experiment using a standard error prone PCR library approach19 are highlighted in yellow.
