Extended Data Fig. 3: Optimization of antibody display in AHEAD.
From: Rapid generation of potent antibodies by autonomous hypermutation in yeast
a, Maps of orthogonal p1 plasmids containing OrthoRep parts driving expression of nanobodies in the first-generation AHEAD 1.0 and improved second-generation AHEAD 2.0 systems. Nb = nanobody, tAHD1 = ADH1 terminator, polyA = polyadenosine tail. b, Increased functional expression of nanobody AT110 using all AHEAD 2.0 parts as determined by FACS. The induced population in AHEAD 2.0 shows an ~25-fold increase in nanobody display levels (determined by mean fluorescence intensity of the cell population) compared to AHEAD 1.0.
