Extended Data Fig. 10: Standard curve of qRT-PCR-derived Ct values versus sample RNA concentration.
From: Accelerated RNA detection using tandem CRISPR nucleases
a) Ct values obtained from qRT-PCR for a dilution series of the positive control RNA provided by the Thermo TaqPath combo kit. The highest concentration of sample added to the reaction is 1000 copies per µl (cp/µl) and lower RNA concentrations were obtained by diluting the concentrated sample two-fold down to ~2 cp/µl. Primers for the N gene of SARS-CoV-2 were used for amplification. Two replicates for each concentration of RNA are plotted as pink squares. b) As in a but with primers for ORF1ab locus of SARS-CoV-2. Replicates are plotted as green triangles. c) As in a but with primers for the S gene of SARS-CoV-2. Replicates are plotted as blue circles.
