Extended Data Fig. 1: Direct activation of TtCsm6 by A4>P oligonucleotides, related to Fig. 1 and Fig. 2.
From: Accelerated RNA detection using tandem CRISPR nucleases
Direct activation of TtCsm6 with varying concentrations of the A4>P oligonucleotide (tetraadenylate with 2´,3´-cyclic phosphate). Mean fluorescence intensity with error bars indicating s.e.m. (n = 3) is shown in arbitrary fluorescence units (AU). The schematic above the graph is a representation of A4>P, with adenosines numbered A1-A4 from 5´ to 3´. Controls lacking TtCsm6 activator (0 µM A4>P) and containing only the fluorescent reporter (Reporter only) are shown for comparison. b) As in a, but with a single-fluoro A4>P oligonucleotide. The nucleotide bearing the 2´-F modification is colored pink in the schematic above the graph. c) As in a, but with a single deoxy A4>P oligonucleotide. The nucleotide bearing the 2´-H modification is colored white in the schematic above the graph. Controls shown in a are overlaid in b and c since all experiments in this figure were run in parallel.
