Extended Data Fig. 9: Increasing cellular crotonylation level compromises EB1 localization to mitotic spindle in mouse gastric organoids.
From: Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis
(a) Cartoon presentation of generation of mouse-derived gastric organoids. The organoid model provides a unique platform to delineate cellular dynamics during cell division in native tissue. (b) Representative immunofluorescence images of EB1 localization in mitotic cells in gastric organoids derived from wild-type mice. Gastric organoids were fixed and stained with EB1 (red), DM1A (green), and DAPI (blue). Scale bar, 10 μm. (c) Representative immunofluorescence images of EB1 localization in mitotic cells in gastric organoids derived from wild-type and TIP60-KI mice. Scale bar, 10 μm. (d) Statistical analyses of EB1 fluorescence intensity in mitotic spindle in c. Wild-type, n = 24; TIP60 KI, n = 24. Data represent mean ± s.e.m. from three independent experiments. Statistical significance was determined by two-sided t-test (***p < 0.0001). Related to Fig. 6.
