Extended Data Fig. 4: Verification of ‘pseudo’ plasmids formed by aberrant recombination events in S. stipitis.
From: A repackaged CRISPR platform increases homology-directed repair for yeast engineering
a, Transformation of E. coli BW25141 with the five plasmids isolated from S. stipitis variants. b, Enzyme digestion verification of pGAOU-X vectors isolated from E. coli DH5α. Lane 1: M, GeneRuler 1 kb DNA Ladder. Lanes 2 and 3: pGAOU-1 isolates. Lanes 4 and 5: pGAOU-2 isolates. Lanes 6 and 7: pGAOU-3 isolates. Individual restriction digestions for each biological sample were performed once. c, Vector maps of the isolated pGAOU-X plasmids. d, Transformation of UC7 with pGAOU-2 and pGAOU-3. Plates were observed under a DR46B transilluminator.
