Extended Data Fig. 2: TmcA can bind with 2-hydroxyisobutyryl-CoA.
From: TmcA functions as a lysine 2-hydroxyisobutyryltransferase to regulate transcription
a, Structural modeling of TmcA combining acetyl-CoA. Exhibition the crystal structure of TmcA combining acetyl-CoA by UCSF Chimera. Ac-CoA: acetyl-CoA. Related to Fig. 2a. b, Structural modeling of TmcA combining 2-hydroxyisobutyryl-CoA. Using Autodock modes the binding of 2-hydroxyisobutyryl-CoA with TmcA and exhibits the crystal structure by UCSF Chimera. Hib-CoA: 2-hydroxyisobutyryl-CoA. c, Structural modeling of TmcA combining Hib-CoA under different setting of Box. Small box:100 Å × 60 Å × 60 Å, large box:120 Å × 80 Å × 80 Å. d, The hydrogen bonds and hydrophobic interaction between TmcA and Hib-CoA are shown by LigPlot+ with large box. e-f, ITC analysis of the affinity of recombinant W504A TmcA mutant with Ac-CoA and Hib-CoA separately, and each group had three biological repetitions, each group had three biological repetitions with similar result.
