Extended Data Fig. 4: Characterization of GPX4R152H in cells and in vitro.
From: Characterization of a patient-derived variant of GPX4 for precision therapy
a, HT1080 overexpressing exogenous WT or R152H GFP-GPX4 and a control line were tested for RSL3, ML162, and IKE sensitivity. Data are plotted as means ± SD, n = 3 biologically independent samples. b-c, SDS-PAGE gel of His-tagged GPX4U46C and GPX4U46C_R152H as stained by Coomasie Blue. Biologically independent duplicate experiments were performed and imaged. d-e, Distances between the catalytic triad in R152H variant were measured and labeled as compared to GPX4U46C (PDB:2OBI). f, Shift of Lys48 away from the active site in the GPX4R152H was plotted.
