Extended Data Fig. 6: Single particle cryo-EM analysis of the GudB6-GltA2B2. | Nature Chemical Biology

Extended Data Fig. 6: Single particle cryo-EM analysis of the GudB6-GltA2B2.

From: A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis

Extended Data Fig. 6

(a) The particles were iteratively picked from selected micrographs and classified. All non-ice particles were carried forward and subjected to a two-class 3D auto refinement in cisTEM using the preliminary GudB6-GltA2B2 reference. This yielded one noise class carrying unaligned particles and high frequency noise, and one class representing clear density for the GudB6-GltA2B2 species. This class was refined using auto and manual methods in cisTEM, with C2 symmetry applied. The number of particles and the estimated resolution are indicated. (b) FSC curves for the refined GudB6-GltA2B2 map. (c) Angular distribution plot and local resolution estimation (Relion3.1.2).

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