Extended Data Fig. 5: CaR-ESI-MS screening ganglioside-containing nanodiscs against RBD.
From: Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
a, ESI mass spectrum acquired in negative ion mode for an aqueous ammonium acetate solution (100 mM, 25 °C and pH 7.4) of RBD (10 µM) and 5 µM ganglioside-containing ND (1% GM1, GM2, GM3, GD1a, GD2, and GT1b, each at approximately 10 μM). b, CID mass spectrum acquired for ions centered at m/z 3,540, with a window width of 100 m/z units (the region highlighted in light blue in (a)) using a Trap collision energy of 50 V. c, CID mass spectrum acquired in negative ion mode for ions centered at m/z 3,540, with a window width of 100 m/z units, produced from an aqueous ammonium acetate solution (100 mM, 25 °C and pH 7.4) of and 10 µM ganglioside-containing ND; the Trap collision energy was 50 V.
