Extended Data Fig. 7: Representative flow cytometry gating strategy and histogram for Fig. 4.
From: Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
a-c, Side scatter (SSC) versus forward scatter (FSC) was used to discriminate against dead cells (SSC-A) and doublets (SSC-W). The singlets were then plotted in a histogram and the mean fluorescence intensity (MFI) was determined. Gating panels corresponding to Fig. 4a (a), 4b (b) and 4c (c). d, Representative gating strategy for Fig. 4d to determine % GFP+ cells in the pseudoviral infection assays when treated with 3FNeu5Ac. e, Representative flow cytometry gating and histogram for ACE2 binding in 3FNeu5Ac treated cells. This strategy corresponds to Fig. 4e.
