Extended Data Fig. 8: Representative flow cytometry gating strategy and histogram for Fig. 5.

a, b, d, f, Side scatter (SSC) versus forward scatter (FSC) was used to discriminate against dead cells (SSC-A) and doublets (SSC-W). The singlets were then plotted in a histogram and the mean fluorescence intensity (MFI) was determined. Gating strategy of Genz-123346 treated cells (a, b) and UGCG⁺ or UGCG^-/- HEK293 ACE2 cells (d, e, f) used to plot Fig. 5b(a), 5c (b), 5f (d), 5g (e), and 5h (f). c,g, Representative gating strategy for Fig. 5d(c) and 5i (g) to determine % GFP⁺ cells in the pseudoviral infection of HEK293 ACE2 UGCG⁺ and UGCG^-/- cells.