Fig. 2: Screening of natural N-glycan libraries against RBD.
From: Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
a,c, CaR-ESI-MS screening results obtained for aqueous ammonium acetate solutions (100 mM, pH 6.9, 37 °C) of RBD (10 μM), P dimer of the Saga strain (Pref, 4 μM) and the N-glycan library from lung (200 μg ml–1) (a) and intestinal (125 μg ml–1) (c) tissues. Ions with an m/z of 3,000–4,000 were subjected to HCD using a collision energy of 50 V. b,d, Chromatograms of 2-aminobenzamide (2-AB)-labeled N-glycans released from lung (b) and intestinal (d) tissues acquired using HILIC with fluorescence detection. The relative abundances of the Neu5Ac content of the N-glycans are indicated graphically. e, Heat maps of relative affinities of ligands identified by CaR-ESI-MS screening at 37 °C; ND, not detected.
