Fig. 6: Pharmacological abrogation of glycolipids decreases authentic SARS-CoV-2 viral infection.
From: Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
a,b, SARS-CoV-2 infection by two authentic SARS-CoV-2 viral strains, VIDO and 72b Calg, in ACE2+ HEK293 cells after GENZ-123346 treatment (a) or in UGCG+ or UGCG–/– ACE2+ HEK293 cells (b). Shown is the viral titer of n = 3 biological replicates with 95% CI error bars. c, Infection of GENZ-123346-treated human primary nasal epithelial cells by 72b Calg virus. Open and solid symbols represent n = 2 biological replicates each containing n = 2 technical replicates at 0.1 and 0.5 multiplicity of infection (MOI). Viral titer was quantified by a plaque-forming unit (PFU) assay. Error bars represent 95% CI. Statistical significance was calculated using two-tailed unpaired Student’s t-test (a–c). Graphs were plotted using GraphPad Prism 8; NS, not significant.
