Extended Data Fig. 2: CaR-ESI-MS screening against defined glycan libraries at 25 °C.
From: Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
Normalized abundances of released glycans from the SARS-CoV-2 RBD by CaR-ESI-MS at 25 °C. Summary of the charge-normalized (relative to 71) abundances of released ligands measured by CaR-ESI-MS screening of Library A – O against SARS-CoV-2 RBD. Measurements were performed in negative ion mode with a UHMR Orbitrap mass spectrometer at an HCD energy of 50 V. Aqueous ammonium acetate (100 mM, pH 6.9) solutions of SARS-CoV-2 RBD (10 μM) and glycan library (containing 50 nM of each glycan) at were used for screening. The different classes of oligosaccharides are distinguished by colour: red - Lewis antigens (1–14); yellow - blood group A antigens (15–20); orange - blood group B antigens (21–26); green - blood group H antigens (27–32); blue - sulfated compounds (34–38) and HS oligosaccharides (39–41), light blue - antigen-related glycans (42–50), purple - globo (51–60), pink - ganglioside oligosaccharides (61–76), white – HMOs and other glycans (77–128), light grey - Neu5Acα2-6-linked oligosaccharides (129–132), and dark grey – rhamnose-containing compounds (133–139). NA, not applicable. Asterisk indicates that the relative abundances (in CaR-ESI-MS) were estimated from their relative (to 69) Kd. Black-outlined white-filled circles represent data points. Data represent means ± s.d., n = 3 independent experiments.
