Extended Data Fig. 5: Ligand binding pocket of S1PR1.
From: Structural basis of sphingosine-1-phosphate receptor 1 activation and biased agonism
a, Cross section of a hydrophobicity analysis in the S1P-bound S1PR1 structure. b, An electrostatic potential surface analysis of the S1P-binding pocket. c, Mutant study to assess ligands response by the NanoBiT-Gi dissociation assay. Dashed lines in the mutant panels represent the wild-type (WT) S1PR1 response. Data are presented as mean values ± SEM, n = 3-5. Note that in many data points, error bars are smaller than the size of symbols and thus are not visible. For details of the ∆pEC50, Emax and error values, see Supplementary Table 2.
