Extended Data Fig. 6: Mutant studies assessing ligand recognition.
From: Structural basis of sphingosine-1-phosphate receptor 1 activation and biased agonism
a, Surface expression of the S1PR1 mutants analyzed by the flow cytometry. b, c, Pharmacological parameters for the Gi-coupling activity analyzed by the NanoBiT-Gi dissociation assay. For the individual experiments performed in parallel, data were normalized to the wild-type (WT) S1PR1 and presented as surface expression level (a), Emax (b) and ∆pEC50 (c). Data are presented as mean values ± SEM, n = 3-5 (dots). NA, parameter not available owing to a lack of ligand response. Mutants with lowered surface expression levels (E1213.29A, W2696.48A and E2947.36×35A) are shown as grey bars.
